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hek 293s gnti cells  (ATCC)


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    Structured Review

    ATCC hek 293s gnti cells
    Hek 293s Gnti Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 780 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hek+293s+gnti/HEK293S+GnTI/pmc13635229-322-11-16
    Average 99 stars, based on 780 article reviews
    hek 293s gnti cells - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Atomic mechanisms of full-length ASC-mediated inflammasome assembly.
    Article Snippet: Sf9 cells (Invitrogen, cat. no. 11496-015) were cultured in Sf-900TM II SFM (Gibco) supplemented with 5% (v/v) heat-inactivated Fetal Bovine Serum (FBS) (Gibco). .. HEK 293S GnTI- cells (ATCC, cat. no. CRL-3022) were grown in Union cell culture medium (Union-Biotech) supplemented with 2% FBS (Gibco). .. HEK293FT cells (Invitrogen, cat. no. R70007) were cultured in Dulbecco’s Modified Eagle Medium high glucose (DMEM) (Gibco) supplemented with GlutaMax (Gibco), NEAA (Gibco), and 10% FBS.

    Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures
    Article Snippet: MCF7 cells were cultured in EMEM medium (ATCC, 30–2003) supplemented with 2 mM L-glutamine, 0.01 mg/ml human recombinant insulin (Sartorious, BE02-033E20), 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 . .. HEK 293S GnTI − (tissue: kidney; Embryo; established by methanesulfonate mutagenesis followed by ricin selection, cell type: epithelial, CRL-3022) cells lacking N -acetyl-glucosaminyltransferase I (GnTI) activity and therefore hybrid and complex N -glycans, were purchased from ATCC (American Type Culture Collection, LGC Standards) and propagated in monolayer culture using standard mammalian cell culture techniques as described in the ATCC protocols. .. HEK 293S GnTI − cells were cultured in the DMEM:F12 medium (ATCC, 30–2006) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 .

    Article Title: Atomic mechanisms of full-length ASC-mediated inflammasome assembly
    Article Snippet: Sf9 cells (Invitrogen, cat. no. 11496-015) were cultured in Sf-900TM II SFM (Gibco) supplemented with 5% (v/v) heat-inactivated Fetal Bovine Serum (FBS) (Gibco). .. HEK 293S GnTI- cells (ATCC, cat. no. CRL-3022) were grown in Union cell culture medium (Union-Biotech) supplemented with 2% FBS (Gibco). .. HEK293FT cells (Invitrogen, cat. no. R70007 ) were cultured in Dulbecco’s Modified Eagle Medium high glucose (DMEM) (Gibco) supplemented with GlutaMax (Gibco), NEAA (Gibco), and 10% FBS.

    Transduction:

    Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family.
    Article Snippet: .. The protein was expressed using baculovirus-mediated transduction of mammalian HEK-293S GnTI- cells (ATCC no. CRL-3022). ..

    Article Title: TRPML2 in distinct states reveals the activation and modulation principles of the TRPML family
    Article Snippet: .. The protein was expressed using baculovirus-mediated transduction of mammalian HEK-293S GnTI- cells (ATCC no. CRL-3022). ..

    Mutagenesis:

    Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures
    Article Snippet: MCF7 cells were cultured in EMEM medium (ATCC, 30–2003) supplemented with 2 mM L-glutamine, 0.01 mg/ml human recombinant insulin (Sartorious, BE02-033E20), 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 . .. HEK 293S GnTI − (tissue: kidney; Embryo; established by methanesulfonate mutagenesis followed by ricin selection, cell type: epithelial, CRL-3022) cells lacking N -acetyl-glucosaminyltransferase I (GnTI) activity and therefore hybrid and complex N -glycans, were purchased from ATCC (American Type Culture Collection, LGC Standards) and propagated in monolayer culture using standard mammalian cell culture techniques as described in the ATCC protocols. .. HEK 293S GnTI − cells were cultured in the DMEM:F12 medium (ATCC, 30–2006) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 .

    Selection:

    Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures
    Article Snippet: MCF7 cells were cultured in EMEM medium (ATCC, 30–2003) supplemented with 2 mM L-glutamine, 0.01 mg/ml human recombinant insulin (Sartorious, BE02-033E20), 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 . .. HEK 293S GnTI − (tissue: kidney; Embryo; established by methanesulfonate mutagenesis followed by ricin selection, cell type: epithelial, CRL-3022) cells lacking N -acetyl-glucosaminyltransferase I (GnTI) activity and therefore hybrid and complex N -glycans, were purchased from ATCC (American Type Culture Collection, LGC Standards) and propagated in monolayer culture using standard mammalian cell culture techniques as described in the ATCC protocols. .. HEK 293S GnTI − cells were cultured in the DMEM:F12 medium (ATCC, 30–2006) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 .

    Activity Assay:

    Article Title: Selective Bioorthogonal Probe for N -Glycan Hybrid Structures
    Article Snippet: MCF7 cells were cultured in EMEM medium (ATCC, 30–2003) supplemented with 2 mM L-glutamine, 0.01 mg/ml human recombinant insulin (Sartorious, BE02-033E20), 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 . .. HEK 293S GnTI − (tissue: kidney; Embryo; established by methanesulfonate mutagenesis followed by ricin selection, cell type: epithelial, CRL-3022) cells lacking N -acetyl-glucosaminyltransferase I (GnTI) activity and therefore hybrid and complex N -glycans, were purchased from ATCC (American Type Culture Collection, LGC Standards) and propagated in monolayer culture using standard mammalian cell culture techniques as described in the ATCC protocols. .. HEK 293S GnTI − cells were cultured in the DMEM:F12 medium (ATCC, 30–2006) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (1 mg/mL) at 37 °C under an atmosphere composed of air, 95% humidity, and 5% CO 2 .



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